BD Biosciences does not make any warranty with respect to the workstation remaining virus free after installation. BD Biosciences is not liable for any claims related to or resulting from buyer/user's failure to install and maintain. The BD FACSDiva Software Reference Manual assumes you have a. BD FACSDiva software.---BD FACSDiva Software.

Summary Here we describe all procedures depicted in more detail. Part 1: How to extract high-resolution dot plots and histograms from FACSDiva Part 2: How to generate histogram overlays (based upon Part 1) Part 3: How to delete quadrant labels (Q1–Q4) within dot plots (based upon Part 1) Part 1: A detailed description of Diva-Fit - how to extract high resolution dot plots and histograms from BD’s FACSDiva software The FACSDiva software itself is unable to export any high-resolution image files; neither copy/paste, nor Export Worksheet Elements result in satisfying image quality.

Interestingly, even the new built-in Save as PDF function of FACSDiva is not able to generate such high resolution, vector-based files – instead it combines the low-resolution raster/pixel graphics in a PDF file. However, FACSDiva supports high-resolution printing, as can easily be seen on any printout generated with the software. In this supplement a detailed description shows how FACSDiva can be “tricked” into producing high-resolution images, using its print functionality and a vector-based PDF file as intermediate step. Below we describe the Diva-Fit procedure step-by-step. With some experience, even the generation of high-resolution histogram overlays will only take a few minutes using Diva-Fit. Our proposed strategy (Diva-Fit) requires several programs to be installed on the computer.

We made sure that, with the exception of FACSDiva, the required software is free of charge and can be easily obtained via download. Administrative rights are necessary to install the software. If you do not have enough rights to install software, ask your local system administrator to install the following software tools. • BD FACSDiva 6.1.2 (any version should work) • PDFCreator 0.9.5 This version or newer is recommended. Alternatively, other PDF printer drivers possibly already installed on the computer could be used (e.g., Adobe Acrobat comes with a PDF printer driver; the free Acrobat Reader does not). However, the method will be different.

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• Adobe Acrobat Reader 9.1.0 We recommend this version or newer. Older versions may not provide all the features required. Step 1: Obtaining and processing data with FACSDiva Prepare your FACS analysis in FACSDiva as usual. This example shows cells expressing a fusion protein consisting of EGFP and Zeo R, rendering the cells EGFP positive and resistant to zeocin (lower panels after selection). Importantly, if a histogram overlay needs to be created, it is essential to make the histograms the same size and to set the scaling of the Y-axis to identical values. To do so, mark both histograms and click the button Make Same Size.